Morphological characterization of virus-like particles in coral reef sponges

Pascelli, Cecilia, Laffy, Patrick W., Kupresanin, Marija, Ravasi, Timothy, and Webster, Nicole S. (2018) Morphological characterization of virus-like particles in coral reef sponges. PeerJ, 6. e5625.

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Abstract

Marine sponges host complex microbial consortia that vary in their abundance, diversity and stability amongst host species. While our understanding of sponge-microbe interactions has dramatically increased over the past decade, little is known about how sponges and their microbial symbionts interact with viruses, the most abundant entities in the ocean. In this study, we employed three transmission electron microscopy (TEM) preparation methods to provide the first comprehensive morphological assessment of sponge-associated viruses. The combined approaches revealed 50 different morphologies of viral-like particles (VLPs) represented across the different sponge species. VLPs were visualized within sponge cells, within the sponge extracellular mesohyl matrix, on the sponge ectoderm and within sponge-associated microbes. Non-enveloped, non-tailed icosahedral VLPs were the most commonly observed morphotypes, although tailed bacteriophage, brick-shaped, geminate and filamentous VLPs were also detected. Visualization of sponge-associated viruses using TEM has confirmed that sponges harbor not only diverse communities of microorganisms but also diverse communities of viruses.

Item ID: 56054
Item Type: Article (Research - C1)
ISSN: 2167-8359
Keywords: Transmission Electron Microscopy, Virus, TEM, VLP, Marine sponges, Great Barrier Reef, GBR, Red Sea
Copyright Information: Copyright 2018 Pascelli et al. Distributed under Creative Commons CC-BY 4.0
Funders: Australian Research Council (ARC), CAPES Fellowship, Science Without Borders
Projects and Grants: ARC Future Fellowship FT120100480
Date Deposited: 07 Nov 2018 07:30
FoR Codes: 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 100%
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