Reprogramming of various cell types to a beta-like state by Pdx1, Ngn3 and MafA

Akinci, Ersin, Banga, Anannya, Tungatt, Katie, Segal, Joanna, Eberhard, Daniel, Dutton, James R., and Slack, Jonathan M.W. (2013) Reprogramming of various cell types to a beta-like state by Pdx1, Ngn3 and MafA. PLoS ONE, 8 (11). e82424.

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Abstract

The three transcription factors, PDX1, NGN3 and MAFA, are very important in pancreatic development. Overexpression of these three factors can reprogram both pancreatic exocrine cells and SOX9-positive cells of the liver into cells resembling pancreatic beta cells. In this study we investigate whether other cell types can be reprogrammed. Eight cell types are compared and the results are consistent with the idea that reprogramming occurs to a greater degree for developmentally related cells (pancreas, liver) than for other types, such as fibroblasts. Using a line of mouse hepatocyte-derived cells we screened 13 compounds for the ability to increase the yield of reprogrammed cells. Three are active and when used in combination they can increase the yield of insulin-immunopositive cells by a factor of six. These results should contribute to the eventual ability to develop a new cure for diabetes based on the ability to reprogram other cells in the body to a beta cell phenotype.

Item ID: 55339
Item Type: Article (Research - C1)
ISSN: 1932-6203
Copyright Information: © 2013 Akinci et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funders: National Institutes of Health (NIH)
Projects and Grants: NIH Grant R01DK080747
Date Deposited: 17 Oct 2018 02:26
FoR Codes: 06 BIOLOGICAL SCIENCES > 0699 Other Biological Sciences > 069999 Biological Sciences not elsewhere classified @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 20%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 20%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920104 Diabetes @ 60%
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