Basic histological techniques for planarians

Winsor, Leigh, and Sluys, Ronald (2018) Basic histological techniques for planarians. In: Rink, Jochen C., (ed.) Planarian Regeneration: methods and protocols. Methods in Molecular Biology, 1774 . Humana Press, New York, NY, USA, pp. 285-351.

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Abstract

The chapter provides a concise account of collecting techniques, and basic histological techniques for investigating planarians, intended for use by practicing scientists and postgraduate students new to the field. Selected hands-on methods are described that will lead to well-stained preparations suitable for light microscopic examination for subsequent taxonomic identification or general investigations into planarian functional cytology and histology. Major equipment items that are minimal essential requirements of a functional histological laboratory are indicated. The chapter includes sectioning and staining troubleshooting guides that will be very helpful for those who must work in the laboratory without the opportunity to consult qualified and experienced technicians or microscopists.

Item ID: 55322
Item Type: Book Chapter (Reference)
ISBN: 978-1-4939-7800-7
ISSN: 1064-3745
Keywords: Platyhelminthes, Tricladida, Planarians, collecting techniques, histological techniques, fixation, paraffin embedding, serial sectioning, staining methods
Copyright Information: The work is subject to copyright.
Date Deposited: 19 Nov 2018 01:01
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060301 Animal Systematics and Taxonomy @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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