Are 3 minutes good enough for obtaining baseline physiological samples from teleost fish?

Lawrence, Michael J., Jain-Schlaepfer, Sofia, Zolderdo, Aaron J., Algera, Dirk A., Gilmour, Kathleen M., Gallagher, Austin J., and Cooke, Steven J. (2018) Are 3 minutes good enough for obtaining baseline physiological samples from teleost fish? Canadian Journal of Zoology, 96 (7). pp. 774-786.

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Abstract

A prerequisite to studying the physiological status of wild animals is the ability to obtain blood samples that reflect the condition prior to capture or handling. Based on research in avian taxa, it is recommended that such samples be obtained within 3 min of capture; however, this guideline has not been validated in wild teleosts. The present study addresses the time course of physiological changes in a number of blood metrics across six species of freshwater fish. Fishes were caught using a standardized angling protocol and held in a water-filled trough prior to the collection of a blood sample, via caudal phlebotomy, between 0.5 and 11 min after capture. Changes in whole-blood glucose and lactate concentrations, hematocrit, and plasma cortisol concentrations were assessed. Change-point analyses indicated that blood lactate concentrations and hematocrit did not deviate from baseline values until similar to 2-5 min of handling for all species, whereas blood glucose concentrations generally did not deviate significantly from baseline over the 11 min test period. In all species, plasma cortisol concentrations began to increase above baseline between similar to 4 and 8 min after capture. Thus, to ensure that blood samples are representative of baseline conditions across multiple metrics, we recommend that sampling be limited to less than 2 min in teleost fishes.

Item ID: 55187
Item Type: Article (Research - C1)
ISSN: 1480-3283
Keywords: stress, cortisol, field endocrinology, glucose, lactate, blood sampling, hematocrit, 3 min rule, wild animals, teleost, largemouth bass, Microptents salmoides, smallmouth bass, Micropterus dolomieu, rock bass, Ambloplites rupestris, pumpkinseed, Lepomis gibbosus, bluegill, Lepomis macrochirus, northern pike, Esox lucius
Copyright Information: Copyright remains with the author(s) or their institution(s).
Funders: Natural Sciences and Engineering Research Council of Canada (NSERC), James Cook University (JCU), Queen Elizabeth II Ontario Graduate Scholarship, Beneath the Waves Inc., Canada Research Chairs Program
Date Deposited: 22 Aug 2018 09:46
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060806 Animal Physiological Ecology @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960807 Fresh, Ground and Surface Water Flora, Fauna and Biodiversity @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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