Successful cryopreservation of African wild dog (Lycaon pictus) spermatozoa: towards developing the frozen zoo

Van den Berghe, F., Paris, M.C.J., Sarnyai, Z., Briggs, M.B., Farstad, W.K., and Paris, D.B.B.P. (2017) Successful cryopreservation of African wild dog (Lycaon pictus) spermatozoa: towards developing the frozen zoo. In: [Presented at the European Association of Zoos and Aquariums Annual Conference]. From: EAZA 2017: European Association of Zoos and Aquariums Annual Conference, 19-23 September 2017, Emmen, The Netherlands.

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Abstract

Sperm freezing and artificial insemination can aid species management and conservation of the African wild dog (Lycaon pictus). Freezing attempts have previously been unsuccessful with sperm motility dropping to nearly 0% within 2 h of thawing. We examined the quality of wild dog spermatozoa subjected to 2 routine canine cryopreservation protocols: 1) 1-step dilution in TRIS-egg yolk extender containing 8% glycerol and 20% egg yolk; and 2) 2-step dilution in TRIS-egg yolk extender to a final concentration of 5% glycerol, 20% egg yolk and 0.5% Equex STM. Protocol 2 showed a significantly higher post-thaw viability, acrosome integrity and longevity of spermatozoa with motility present for up to 8 h after thawing; making it suitable for sperm banking and downstream use in species management by artificial insemination for the first time.

Item ID: 54252
Item Type: Conference Item (Abstract / Summary)
Keywords: African wild dog; Lycaon pictus; sperm bank; cryopreservation; Equex STM; wildlife; conservation; biobanking
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Date Deposited: 18 Jul 2018 00:17
FoR Codes: 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060803 Animal Developmental and Reproductive Biology @ 50%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death @ 25%
05 ENVIRONMENTAL SCIENCES > 0502 Environmental Science and Management > 050202 Conservation and Biodiversity @ 25%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 25%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 25%
96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960899 Flora, Fauna and Biodiversity of Environments not elsewhere classified @ 50%
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