Pena, Santiago T., Gummow, Bruce, Parker, Anthony J., and Paris, Damien B.B.P. (2022) Boar spermatozoa maintains DNA integrity after cryopreservation using different concentrations of glycerol. Philippine Journal of Veterinary Medicine, 59 (2). pp. 164-173.

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Abstract

Despite its potential benefits, the application of frozen-thawed boar spermatozoa in commercial pig production is still very limited. Cryopreservation could induce sperm DNA damage causing reduced fertility and early embryo loss. Glycerol is a popular cryoprotectant used to improve motility and plasma membrane integrity during sperm freezing but the optimal concentration needed to prevent DNA damage without causing cell toxicity is unknown. Thus, the aim of this study was to determine the cryoprotective effect of different concentrations of glycerol on DNA integrity and motility of frozen-thawed boar spermatozoa. Using the TUNEL assay and flow cytometry, there was no significant difference in the percentage of sperm DNA damage between fresh or frozen-thawed sperm at 3%, 6%, and 8% glycerol (1.9 ± 0.4 vs. 3.5 ± 0.8 vs. 2.8 ± 0.5 vs. 3.0 ± 0.8% respectively; P > 0.05). While total and progressively motile spermatozoa were higher in fresh samples, other CASA motion parameters such as straight-line velocity, average path velocity, straightness, and linearity were generally higher in 3% and 6% glycerol-frozen than fresh samples (P ≤ 0.05). At the concentrations tested, glycerol appears to protect DNA integrity of boar spermatozoa during cryopreservation while slightly enhancing some sperm motility parameters.

Item ID:	54232
Item Type:	Article (Research - C1)
ISSN:	0031-7705
Keywords:	boar, pig, sperm, DNA damage, glycerol, sperm motility, cryopreservation, freezing, TUNEL, CASA
Related URLs:	Publisher
Copyright Information:	© 2022 College of Veterinary Medicine, University of the Philippines Los Baños
Funders:	AusAID, James Cook University
Projects and Grants:	Australian Awards Scholarship, JCU Development Grant
Date Deposited:	03 May 2023 03:48
FoR Codes:	07 AGRICULTURAL AND VETERINARY SCIENCES > 0702 Animal Production > 070206 Animal Reproduction @ 50% 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060103 Cell Development, Proliferation and Death @ 30% 10 TECHNOLOGY > 1001 Agricultural Biotechnology > 100199 Agricultural Biotechnology not elsewhere classified @ 20%
SEO Codes:	83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8303 Livestock Raising > 830308 Pigs @ 40% 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 30% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 30%
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