A comparative assessment of the activity and structure of phlorotannins from the brown seaweed Carpophyllum flexuosum

Zhang, Rui, Yuen, Alexander K.L., Magnusson, Marie, Wright, Jeffrey T., de Nys, Rocky, Masters, Anthony F., and Maschmeyer, Thomas (2018) A comparative assessment of the activity and structure of phlorotannins from the brown seaweed Carpophyllum flexuosum. Algal Research, 29. pp. 130-141.

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Abstract

The extraction and antioxidant activity of phlorotannins from the brown seaweeds Carpophyllum flexuosum, Carpophyllum plumosum and Ecklonia radiata was investigated to identify an optimised extraction process for novel anti-oxidant extracts. Subsequently, the composition of the most active phlorotannin extracts was determined. Microwave assisted extraction (MAE) using water was the most efficient extraction process with shorter processing times and a higher purity product than obtained with any of the other methods tested. MAE resulted in the fast, effective decomposition of the cellular structure, as identified through scanning electron microscopy (SEM), and this related directly to the efficiency of extraction. Phlorotannins extracted from C. flexuosum by MAE had the strongest antioxidant activity (62.1 mg gallic acid equivalents (GAE)/g dw of seaweed) and more than 5.5-fold greater 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability than ascorbic acid after 7-day incubation periods at 30°C and at 60°C. Six major chemical species of phlorotannin, belonging to the fuhalol group, were identified within the MAE extract using NMR and HPLC-MS. The results confirm phlorotannins from C. flexuosum to be promising natural, bio-derived and bio-compatible antioxidants, while identifying the most effective method to extract the constituents and retain antioxidant activity.

Item ID: 53722
Item Type: Article (Research - C1)
ISSN: 2211-9264
Keywords: brown seaweed, phlorotannis, antioxidants activity, microwave assisted extraction, Ecklonia radiata, Carpophyllum plumosum
Funders: University of Sydney, Australian Government's Cooperative Research Centre Scheme (CRCS), Science and Industry Endowment Fund (SIEF)
Projects and Grants: CRCS DP109602, SIEF RP03-028
Date Deposited: 24 Aug 2018 02:59
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 30%
10 TECHNOLOGY > 1003 Industrial Biotechnology > 100302 Bioprocessing, Bioproduction and Bioproducts @ 70%
SEO Codes: 82 PLANT PRODUCTION AND PLANT PRIMARY PRODUCTS > 8203 Industrial Crops > 820399 Industrial Crops not elsewhere classified @ 100%
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