A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog(Lycaon pictus)

Van den Berghe, Femke, Paris, Monique Christina Johanna, Briggs, Michael Brent, Farstad, Wenche Kristin, and Paris, Damien Boyd Bertrand Paul (2018) A two-step dilution tris-egg yolk extender containing Equex STM significantly improves sperm cryopreservation in the African wild dog(Lycaon pictus). Cryobiology, 80. pp. 18-25.

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DOI: 10.1016/j.cryobiol.2017.12.095

View at Publisher Website: https://doi.org/10.1016/j.cryobiol.2017....

1094

Abstract

Conservation management of endangered African wild dogs (AWD; Lycaon pictus) can benefit greatly from development of sperm freezing and artificial insemination. Previous freezing attempts yielded nearly 0% motile sperm within 2 h of thawing. In this study, two canine freezing protocols were tested: Protocol 1: a one-step dilution in TRIS-20% egg yolk containing 8% glycerol; and Protocol 2: a two-step dilution in TRIS-20% egg yolk containing a final extender concentration of 5% glycerol and 0.5% Equex STM, coupled with a TRIS-citratefructose thawing solution. Semen was collected by electroejaculation from n=24 AWDs, of which eight ejaculates of sufficient quality (four good quality with initial sperm motility of 75.0 ± 4.4% and four poor quality; showing rapid decrease in sperm motility to 3.3 ± 3.3% prior to freezing) were frozen. For good quality samples, motility and sperm motility index persisted for up to 8 h for Protocol 2, and was higher between 2 and 6 h after thawing with a decrease from 4 h of incubation. Motility dropped to nearly 0% after 2 h incubation for Protocol 1. Viability was higher for Protocol 2 throughout the 8 h of incubation, with a decrease after 6 h, compared to 4 h for Protocol 1. Acrosome integrity was higher for Protocol 2 throughout post-thaw incubation, with a decrease after 2 h for both protocols. Protocols did not differ in normal sperm morphology or DNA integrity. Poor quality samples yielded similar results, except for acrosome integrity, which declined for Protocol 2. In conclusion, a two-step dilution in TRIS-egg yolk glycerol extender containing Equex STM yields significantly improved post-thaw quality and longevity of AWD spermatozoa, making it suitable for sperm banking and artificial insemination initiatives.


Item ID:	52999
Item Type:	Article (Research - C1)
ISSN:	1090-2392
Keywords:	African wild dog; Lycaon pictus; sperm bank; cryopreservation; Equex STM; wildlife; conservation; biobanking
Additional Information:	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.
Funders:	Morris Animal Foundation (MAF), Roger Williams Park Zoo, Fresno Chaffee Zoo, James Cook University, IBREAM
Projects and Grants:	MAF D15ZO-053
Date Deposited:	28 Mar 2018 03:46
FoR Codes:	31 BIOLOGICAL SCIENCES > 3109 Zoology > 310903 Animal developmental and reproductive biology @ 50% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310102 Cell development, proliferation and death @ 25% 41 ENVIRONMENTAL SCIENCES > 4104 Environmental management > 410401 Conservation and biodiversity @ 25%
SEO Codes:	96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960899 Flora, Fauna and Biodiversity of Environments not elsewhere classified @ 50% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 25% 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 25%
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