Irisin treatment improves healing of dystrophic skeletal muscle

Reza, Musarrat Maisha, Sim, Chu Ming, Subramaniyam, Nathiya, Ge, Xiaojia, Sharma, Mridula, Kambadur, Ravi, and McFarlane, Craig (2017) Irisin treatment improves healing of dystrophic skeletal muscle. Oncotarget, 8. pp. 98553-98566.

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Background: Irisin is an exercise induced myokine that is shown to promote browning of adipose tissue and hence, increase energy expenditure. Furthermore, our unpublished results indicate that Irisin improves myogenic differentiation and induces skeletal muscle hypertrophy. Since exercise induced skeletal muscle hypertrophy improves muscle strength, we wanted to investigate if ectopic injection of Irisin peptide improves skeletal muscle function in a mouse model of muscular dystrophy. This utility of Irisin peptide is yet to be studied in animal models.

Methods: In order to test this hypothesis, we expressed and purified recombinant murine Irisin peptide from E. coli. Three- to six-week-old male mdx mice were injected IP with either vehicle (dialysis buffer) or Irisin recombinant peptide for two or four weeks, three times-a-week.

Results: Irisin injection increased muscle weights and enhanced grip strength in mdx mice. Improved muscle strength can be attributed to the significant hypertrophy observed in the Irisin injected mdx mice. Moreover, Irisin treatment resulted in reduced accumulation of fibrotic tissue and myofiber necrosis in mdx mice. In addition, Irisin improved sarcolemmal stability, which is severely compromised in mdx mice.

Conclusion: Irisin injection induced skeletal muscle hypertrophy, improved muscle strength and reduced necrosis and fibrotic tissue in a murine dystrophy model. These results demonstrate the potential therapeutic value of Irisin in muscular dystrophy.

Item ID: 52331
Item Type: Article (Research - C1)
ISSN: 1949-2553
Keywords: skeletal muscle; systrophy; FNDC5; irisin; sarcolemmal stability
Additional Information:

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funders: National Medical Research Council of Singapore (NMRC), Agency for Science, Technology and Research, Singapore, Nanyang Technological University, Singapore
Date Deposited: 05 Feb 2018 05:35
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310910 Animal physiology - systems @ 70%
31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310102 Cell development, proliferation and death @ 15%
42 HEALTH SCIENCES > 4207 Sports science and exercise > 420702 Exercise physiology @ 15%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970111 Expanding Knowledge in the Medical and Health Sciences @ 50%
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