Physiological responses and ovarian development of female Chinese mitten crab Eriocheir sinensis subjected to different salinity conditions

Long, Xiaowen, Wu, Xugan, Zhao, Lei, Ye, Haihui, Cheng, Yongxu, and Zeng, Chaoshu (2018) Physiological responses and ovarian development of female Chinese mitten crab Eriocheir sinensis subjected to different salinity conditions. Frontiers in Physiology, 8. 1072.

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Abstract

Salinity plays a key role affecting ovarian development, osmoregulation and metabolism of female Chinese mitten crab, Eriocheir sinensis during reproductive migration. In this study, female E. sinensis after their puberty molt were subjected to four salinities of 0, 6, 12, and 18% for 40 days to investigate the salinity effects on their ovarian development as well as a range of important physiological parameters. Elevated salinity accelerated the ovarian development with ovigerous crabs found at salinity treatments of 12 and 18% despite no copulation had occurred. Meanwhile the survival rate of female crabs showed a decreasing trend with increasing salinity. Higher salinity also led to increased hemolymph Na+, K+, Ca2+, Cl-, and Mg2+ concentrations. The 6% treatment had the highest contents of hemolymph total and major free amino acids while the Na+/K+ -ATPase activity in the posterior gills was the lowest among treatments. Total n-3 polyunsaturated fatty acids (Sigma n-3PUFA) and n-3/n-6 PUFA ratio in the anterior gills showed a decreasing trend with salinity while 18% had the highest Sigma PUFA and Sigma n-6PUFA. The Sigma n-3PUFA content and n-3/n-6 PUFA ratio of the posterior gills showed a fluctuating pattern and the highest value was detected at 0%, while an increasing trend was found for the Sigma n-6PUFA with increasing salinity. The hemolymph glucose showed a decreasing trend with increasing salinity and the highest total cholesterol in hemolymph was detected at 12%. The 18% treatment had the highest levels of hemolymph gamma-glutamyltransferase, alkaline phosphatase and acid phosphatase, as well as glucose, urea and acid phosphatase in hepatopancreas while the highest hemolymph superoxide dismutase and malondialdehyde were detected at 0%. Overall, the results showed that salinity increase from freshwater to brackish conditions led to lower metabolism, accelerated ovarian development, and the appearance of ovigerous crabs without copulation in female E. sinensis post puberty molt.

Item ID: 52144
Item Type: Article (Research - C1)
ISSN: 1664-042X
Keywords: Eriocheir sinensis, females, salinity, ovarian development, physiological responses
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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Funders: Shanghai Municipal Science and Technology Commission (SMSTC), Science and Technological Cooperation (STC), Natural Science Foundation of China (NSFC), Shanghai Municipal Education Committee (SMEC), Shanghai Agriculture Committee (SAC), Shanghai Engineering and Technology Center (SETC), Ministry of Agriculture, China (MA)
Projects and Grants: SMSTC project 13320502100, SMSTC profect 13231203504, STC project 2014DFT30270, NSFC general project no. 31572630, SMEC 2015-62-0908, SAC extension project (2015-1-7), SETC Capacity Promotion Project 16DZ2281200, SETC Capacity Promotion Project 13DZ2280500, MA special fund CARS-46
Date Deposited: 24 Jan 2018 07:30
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 70%
06 BIOLOGICAL SCIENCES > 0606 Physiology > 060603 Animal Physiology Systems @ 30%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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