Response of mixed methanotrophic consortia to different methane to oxygen ratios

Chidambarampadmavathy, K., Karthikeyan, O.P., Huerlimann, R., Maes, G.E., and Heimann, K. (2017) Response of mixed methanotrophic consortia to different methane to oxygen ratios. Waste Management, 61. pp. 220-228.

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Abstract

Methane (CH4) and oxygen (air) concentrations affect the CH4 oxidation capacity (MOC) and mixed methanotrophic community structures in compost (fresh) and landfill (age old) top cover soils. A change in the mixed methanotrophic community structure in response has implications for landfill CH4 bio-filter remediation and possible bio-product outcomes (i.e., fatty acid methyl esters (FAME) content and profiles and polyhydroxybutyrate (PHB) contents). Therefore the study aimed to evaluate the effect of variable CH4 to oxygen ratios (10–50% CH4 in air) on mixed methanotrophic community structures enriched from landfill top cover (LB) and compost soils (CB) and to quantify flow on impacts on MOC, total FAME contents and profiles, and PHB accumulation. A stable consortium developed achieving average MOCs of 3.0 ± 0.12, 4.1 ± 0.26, 6.9 ± 0.7, 7.6 ± 1.3 and 9.2 ± 1.2 mg CH4 g−1 DWbiomass h−1 in LB and 2.9 ± 0.04, 5.05 ± 0.32, 6.7 ± 0.31, 7.9 ± 0.61 and 8.6 ± 0.48 mg CH4 g−1 DWbiomass h−1 in CB for a 20 day cultivation period at 10, 20, 30, 40 and 50% CH4, respectively. CB at 10% CH4 had a maximal FAME content of 40.5 ± 0.8 mg FAME g−1 DWbiomass, while maximal PHB contents (25 mg g−1 DWbiomass) was observed at 40% CH4 in LB. Despite variable CH4/O2 ratios, the mixed methanotrophic community structures in both LB and CB were relatively stable, dominated by Methylosarcina sp., and Chryseobacterium, suggesting that a resilient consortium had formed which can now be tested in bio-filter operations for CH4 mitigations in landfills.

Item ID: 51092
Item Type: Article (Research - C1)
ISSN: 1879-2456
Keywords: landfill cover soil, compost soil, methane, biopolymer, Methylosarcina, Chryseobacterium
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A version of this publication was included as Chapter 3 of the following PhD thesis: Chidambaram Padmavathy, Karthigeyan (2017) Response of methanotroph-heterotroph consortia to different environmental factors. PhD thesis, James Cook University, which is available Open Access in ResearchOnline@JCU. Please see the Related URLs for access.

Funders: Advanced Manufacturing Cooperative Research Centre (AMCRC)
Projects and Grants: AMCRC grant number 2.3.4
Date Deposited: 10 Oct 2017 02:18
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060503 Microbial Genetics @ 50%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060504 Microbial Ecology @ 50%
SEO Codes: 96 ENVIRONMENT > 9606 Environmental and Natural Resource Evaluation > 960699 Environmental and Natural Resource Evaluation not elsewhere classified @ 100%
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