Xie, Huixu, Wang, Jianyun, He, Yan, Gu, Zhipeng, Xu, Jia, Li, Longjiang, and Ye, Qingsong (2017) Biocompatibility and safety evaluation of a silk fibroin-doped calcium polyphosphate scaffold copolymer in vitro and in vivo. RSC Advances, 73 (7). pp. 46036-46044.

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Abstract

For the reconstruction of cartilage and bone defects, bone repair scaffolds with porous network structures have been extensively studied. In our previous study, CPP-type bioceramics showed higher compressive strength and enhanced degradation after silk fibroin doping, and SF/CPP could be considered a suitable bioceramic for bone tissue-engineering. The aim of this study was to evaluate the biocompatibility and safety of SF/CPP in vitro and in vivo. The cell biocompatibility was evaluated with regard to the cytotoxicity of the scaffolds using co-culture and MTT tests in vitro. The in vivo biocompatibility of SF/CPP was evaluated by implanting the scaffolds in the subcutaneous and intramuscular regions of experimental animals. We established an experimental animal model to prepare critical-sized cranial defects and evaluated the biodegradability and osteoconductivity of the scaffolds in vivo. The results indicated that the SF/CPP scaffold yielded better biocompatibility and safety performance than the CPP scaffold in vitro and in vivo. Immunohistochemistry staining in vivo for OPN and OCN also indicated that SF/CPP has potential to promote the regeneration of critical-sized cranial defects. The SF/CPP scaffold has good biocompatibility and safety for experimental animals and could also serve as a potential effective bioceramic for a range of bone regeneration applications.

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