Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria

Raina, Jean-Baptiste, Clode, Peta L., Cheong, Soshan, Bougoure, Jeremy, Kilburn, Matt R., Reeder, Anthony, Forêt, Sylvain, Stat, Michael, Beltran, Victor, Thomas-Hall, Peter, Tapiolas, Dianne, Motti, Cherie M., Gong, Bill, Pernice, Mathieu, Marjo, Christopher E., Seymour, Justin R., Willis, Bette L., and Bourne, David G. (2017) Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria. eLife, 6.

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Abstract

Phytoplankton-bacteria interactions drive the surface ocean sulfur cycle and local climatic processes through the production and exchange of a key compound: dimethylsulfoniopropionate (DMSP). Despite their large-scale implications, these interactions remain unquantified at the cellular-scale. Here we use secondary-ion mass spectrometry to provide the first visualization of DMSP at sub-cellular levels, tracking the fate of a stable sulfur isotope (34 S) from its incorporation by microalgae as inorganic sulfate to its biosynthesis and exudation as DMSP, and finally its uptake and degradation by bacteria. Our results identify for the first time the storage locations of DMSP in microalgae, with high enrichments present in vacuoles, cytoplasm and chloroplasts. In addition, we quantify DMSP incorporation at the single-cell level, with DMSPdegrading bacteria containing seven times more 34 S than the control strain. This study provides an unprecedented methodology to label, retain, and image small diffusible molecules, which can be transposable to other symbiotic systems.

Item ID: 50692
Item Type: Article (Research - C1)
ISSN: 2050-084X
Additional Information:

Copyright Raina et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Funders: Australian Research Council (ARC)
Projects and Grants: ARC DE160100636
Date Deposited: 20 Sep 2017 11:05
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060599 Microbiology not elsewhere classified @ 100%
SEO Codes: 96 ENVIRONMENT > 9699 Other Environment > 969999 Environment not elsewhere classified @ 100%
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