Quantitative analysis of species specificity of two anti-parvalbumin antibodies for detecting southern hemisphere fish species demonstrating strong phylogenetic association

Liang, Ji, Tan, Chui Choo, Taylor, Steve L., Baumert, Joseph L., Lopata, Andreas L., and Lee, N. Alice (2017) Quantitative analysis of species specificity of two anti-parvalbumin antibodies for detecting southern hemisphere fish species demonstrating strong phylogenetic association. Food Chemistry, 237. pp. 588-596.

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Abstract

This study aimed to develop a novel approach to determine the correlation between the parvalbumin (PAV) contents and their corresponding immunoreactivity (detectability) in southern hemisphere fish species. The immuno-detected PAV contents of the test fish species were estimated by a quantitative SDS-PAGE. A quantitative Enzyme-Linked ImmunoSorbent Assay (ELISA) was formatted to assess relative immunoreactivity of PAV. Sixteen species (forty-three percent) displayed a positive correlation with the anti-cod PAV polyclonal antibody, but no correlation with the anti-carp PAV monoclonal antibody. There was a strong phylogenetic association of the PAV immunoreactivity. Species from the order of Perciformes showed strong binding with both antibodies; whereas species from Salmoniformes, Ophidiiformes, Scombriformes, Scorpaeniformes, and Tetraodontiformes showed weak or no binding. This approach showed for the first time a statistical correlation between the PAV content and the immunoreactivity and allowed to rank the relative species/order specificity of the two antibodies for the southern hemisphere fish PAV. (C) 2017 Elsevier Ltd. All rights reserved.

Item ID: 50480
Item Type: Article (Refereed Research - C1)
Keywords: Southern hemisphere fish, Parvalbumin, species specificity, order specificity, ELISA, anti-cod parvalbumin antibody, anti-carp parvalbumin antibody
ISSN: 1873-7072
Funders: National Health and Medical Research Council (NHMRC), Australian Research Council (ARC), University of Nebraska-Lincoln (UNL)
Projects and Grants: NHMRC grant 1086656, ARC IC130100021, UNL FARRP
Date Deposited: 20 Sep 2017 09:28
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1101 Medical Biochemistry and Metabolomics > 110106 Medical Biochemistry: Proteins and Peptides (incl Medical Proteomics) @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110701 Allergy @ 50%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920108 Immune System and Allergy @ 80%
92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920406 Food Safety @ 20%
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