Enantioselective reduction of aryl ketones using immobilized cells of Candida viswanathii

Fatima, Yaqoot, Himani, Kansal, Soni, Pankaj, and Banerjee, Uttam C. (2007) Enantioselective reduction of aryl ketones using immobilized cells of Candida viswanathii. Process Biochemistry, 42 (10). pp. 1412-1418.

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Enantioselective reduction of 1-acetonapthone to S(−)-1-(1-naphthyl) ethanol, a key intermediate for the synthesis of HMG Co-A reductase inhibitor, was successfully carried out using immobilized cells of a newly isolated carbonyl reductase producing yeast strain Candida viswanathii MTCC 5158. Calcium alginate (1.5%, w/v) gave the best immobilization efficiency. Among different organic solvents and ionic liquids tried as reaction media, isopropanol gave the best enantioselectivity with moderate conversion. The immobilized cells (100 mg/ml in 50 mM Tris buffer pH 9) showed best results at a substrate concentration of 0.2 mg/ml at 30 °C. After twelve cycles of reaction, no significant decrease in bioreduction efficiency of the immobilized cells was observed as compared to the free cells.

Item ID: 48100
Item Type: Article (Research - C1)
ISSN: 1873-3298
Keywords: immobilized cells; Candida viswanathii; enantioselective reduction; organic solvents
Funders: Government of India, Council of Scientific and Industrial Research, India, National Institute of Pharmaceutical Education and Research (NIPER)
Projects and Grants: NIPER communication number 371
Date Deposited: 27 Apr 2017 04:49
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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