Fatima, Yaqoot, Himani, Kansal, Soni, Pankaj, and Banerjee, Uttam C. (2007) Enantioselective reduction of aryl ketones using immobilized cells of Candida viswanathii. Process Biochemistry, 42 (10). pp. 1412-1418.

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Abstract

Enantioselective reduction of 1-acetonapthone to S(−)-1-(1-naphthyl) ethanol, a key intermediate for the synthesis of HMG Co-A reductase inhibitor, was successfully carried out using immobilized cells of a newly isolated carbonyl reductase producing yeast strain Candida viswanathii MTCC 5158. Calcium alginate (1.5%, w/v) gave the best immobilization efficiency. Among different organic solvents and ionic liquids tried as reaction media, isopropanol gave the best enantioselectivity with moderate conversion. The immobilized cells (100 mg/ml in 50 mM Tris buffer pH 9) showed best results at a substrate concentration of 0.2 mg/ml at 30 °C. After twelve cycles of reaction, no significant decrease in bioreduction efficiency of the immobilized cells was observed as compared to the free cells.

Item ID:	48100
Item Type:	Article (Research - C1)
ISSN:	1873-3298
Keywords:	immobilized cells; Candida viswanathii; enantioselective reduction; organic solvents
Funders:	Government of India, Council of Scientific and Industrial Research, India, National Institute of Pharmaceutical Education and Research (NIPER)
Projects and Grants:	NIPER communication number 371
Date Deposited:	27 Apr 2017 04:49
FoR Codes:	06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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