The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway

Gong, Jie, Huang, Chencui, Shu, Ling, Bao, Chenchang, Huang, Huiyang, Ye, Haihui, Zeng, Chaoshu, and Li, Shaojing (2016) The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway. Scientific Reports, 6. pp. 1-13.

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Abstract

In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean reproduction are poorly understood. In the present study, the RXR mRNA was detected in the ovarian follicular cells of mud crab Scylla paramamosain (SpRXR) and during ovarian maturation, its expression level was found to increase significantly. In vitro experiment showed hat both SpRXR and vitellogenin (SpVg) mRNA in the ovarian explants were significantly induced by 20-hydroxyecdysone (20E) but not methyl farnesoate (MF). However, differing from the in vitro experiment, injection of MF in in vivo experiment significantly stimulated the expressions of SpRXR and SpVg in female crabs at early vitellogenic stage, but the ecdysone and insect juvenile hormone (JH) signaling pathway genes were not induced. The results together suggest that both MF and SpRXR play significant roles in regulating the expression of SpVg and ovarian development of S. paramamosain through their own specific signaling pathway rather than sharing with the ecdysone or the insect JH.

Item ID: 47949
Item Type: Article (Refereed Research - C1)
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ISSN: 2045-2322
Funders: National Natural Science Foundation of China (NNSFC)
Projects and Grants: NSFC No. 31272632, NSFC No. 41476119, NSFC No. 31472261
Date Deposited: 31 Mar 2017 02:37
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 50%
06 BIOLOGICAL SCIENCES > 0608 Zoology > 060803 Animal Developmental and Reproductive Biology @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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