Development and assessment of radioreceptor binding assays for the detection of saxitoxin binding proteins in biological extracts

Robertson, Alison, Negri, Andrew P., Burnell, James N., and Llewellyn, Lyndon E. (2006) Development and assessment of radioreceptor binding assays for the detection of saxitoxin binding proteins in biological extracts. Analytical Biochemistry, 356 (1). pp. 66-75.

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Abstract

Several radioreceptor assays using tritiated saxitoxin ([3H]STX) were developed to identify a suitable primary screening method for the detection and characterization of soluble saxitoxin binding proteins from biological extracts. Assays using anion and cation exchange, protein binding, and traditional charcoal radioreceptor methods were compared with two previously reported formats. A protein binding assay incorporating filters of mixed cellulose esters (MCE) outperformed all other assay strategies with maximal signal, low background, exceptional reproducibility, minimal matrix effects, and high throughput. Binding site titrations verified that an increase in total protein in the assay led to a concomitant linear increase in the amount of specifically bound [3H]STX within the range of 1-90 μg total protein. Saturation binding experiments demonstrated that the binding sites were saturable and that nonspecific binding was linear. The MCE assay was unaffected by 600 mM NaCl and 500 mM KCl. Likewise, minimal variation of specific binding was observed between pH 5 and pH 9, but inhibition was observed below pH 5.

Item ID: 4645
Item Type: Article (Research - C1)
ISSN: 1096-0309
Keywords: saxitoxin; saxitoxin binding protein; saxiphilin; paralytic shellfish toxin; assay; receptor; radioreceptor assay
Date Deposited: 17 Jun 2009 04:39
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060101 Analytical Biochemistry @ 100%
SEO Codes: 92 HEALTH > 9204 Public Health (excl. Specific Population Health) > 920406 Food Safety @ 100%
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