Microsatellite loci from the endemic Southern Ocean octopus Adelieledone polymorpha (Robson, 1930)

Strugnell, Jan M., Allcock, A. Louise, and Watts, Phillip C. (2009) Microsatellite loci from the endemic Southern Ocean octopus Adelieledone polymorpha (Robson, 1930). Molecular Ecology Resources, 9 (3). pp. 1068-1070.

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To determine the pattern of spatial genetic structure in the endemic Southern Ocean octopus Adelieledone polymorpha, microsatellite loci were isolated from partial genomic libraries enriched for repetitive DNA motifs. Seven dinucleotide and two trinucleotide microsatellite loci were isolated successfully and levels of polymorphism were quantified in 34 individuals sampled from the Southern Ocean near South Georgia. No pairs of microsatellite loci were linked significantly; however, one locus deviated (P < 0.05) from Hardy-Weinberg equilibrium. Overall, the nine loci produced between five and 16 alleles, with observed and expected heterozygosities varying between 0.22 and 0.86 and between 0.21 and 0.94 respectively. This is the first description of microsatellite loci from an octopus endemic to the Southern Ocean, and these genetic markers are being used to quantify spatial structure within A. polymorpha.

Item ID: 46359
Item Type: Article (Research - C1)
ISSN: 1755-0998
Keywords: Adelieledone polymorpha, Antarctica, microsatellites, population structure, Southern Ocean
Funders: National Environmental Research Council (NERC), Lloyd’s Tercentenary Fellowship (LTF), Antarctic Science Bursary (ASB), Systematics Association, Edith Mary Pratt Musgrave Fund (EMPMF)
Projects and Grants: NERC AFI NE/C506321/1, CoSyst
Date Deposited: 16 Nov 2016 07:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960808 Marine Flora, Fauna and Biodiversity @ 100%
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