Novel polymorphic microsatellite markers for Panulirus ornatus and their cross-species primer amplification in Panulirus homarus

Delghandi, Madjid, Afzal, Hasifa, Al Hinai, Manal Saif Nasser, Al-Breiki, Rafaida Dhuhai Gharib, Jerry, Dean R., and Dao, Hoc Tan (2016) Novel polymorphic microsatellite markers for Panulirus ornatus and their cross-species primer amplification in Panulirus homarus. Animal Biotechnology, 27 (4). pp. 310-314.

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Abstract

Polymorphic microsatellite loci were isolated for Panulirus ornatus using 454 GS-FLX Titanium pyrosequencing. Fifteen markers containing perfect di-, tri-, tetra-, and penta-nucleotide motifs were consistently co-amplified in five multiplexes in a panel of 91 randomly selected samples. Observed number of alleles varied from 2 to 14 per locus. Observed and expected heterozygosity ranged from 0.090 to 0.79 and 0.08 to 0.87, respectively. Ten loci deviated from Hardy-Weinberg equilibrium after sequential Bonferroni correction. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between 11 loci. The microsatellite markers were also amplified successfully in related Panulirus homarus species with adequate level of polymorphism. The successful cross-species primer amplification of the 15 microsatellites indicates the potential of the developed markers to be transferred to other Panulirus species. The 15 novel microsatellite markers reported in this work add to the previously characterized markers by our group, exhibit adequate levels of polymorphism for wide range of future studies investigating population structure, genetic diversity, and evolutionary relationships among Panulirus species.

Item ID: 45954
Item Type: Article (Refereed Research - C1)
Keywords: 454 sequencing, microsatellites, multiplexing, panulirus, spiny lobster
ISSN: 1532-2378
Funders: Research Council of Oman (TRC), Sultan Qaboos University (SQU)
Projects and Grants: TRC ORG/SQU/EBR/13/027, SQU IG/DVC/CEMB/14/01
Date Deposited: 05 Oct 2016 07:45
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070403 Fisheries Management @ 50%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830101 Aquaculture Crustaceans (excl. Rock Lobster and Prawns) @ 100%
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