Moya, Aurélie, Tambutté, Sylvie, Béranger, Guillaume, Gaume, Béatrice, Scimeca, Jean-Claude, Allemand, Denis, and Zoccola, Didier (2008) Cloning and use of a coral 36B4 gene to study the differential expression of coral genes between light and dark conditions. Marine Biotechnology, 10 (6). pp. 653-663.

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Abstract

This paper aims to validate reference genes for gene expression studies between light and dark conditions in the scleractinian coral Stylophora pistillata for future gene expression studies of the “light-enhanced calcification” phenomenon. For this purpose, we cloned, sequenced, and characterized a candidate reference gene, the 36B4 gene from the coral S. pistillata, and validated 36B4 and β-actin as reference genes. To illustrate the future applications of these reference genes, we tested the dark and light expression of two photosynthetic genes (Rubisco and D1 protein of the photosystem II) and two genes encoding proteins involved in calcium transport for coral calcification (a calcium ATPase and a calcium channel). Results show that both photosynthetic genes are enhanced during the light when standardized against 36B4 and β-actin, whereas the two genes encoding proteins involved in calcium transport are not differentially expressed between light and dark conditions. The characterization of a coral 36B4 and the establishment of such valid reference genes will be useful for future gene expression studies between diverse conditions (aposymbiotic/symbiotic, stress/control, light/dark conditions) in scleractinian corals.

Item ID:	45247
Item Type:	Article (Research - C1)
ISSN:	1436-2236
Keywords:	real-time PCR, coral calcification, stylophora pistillata, acidic ribosomal phosphoprotein P0, 36B4
Funders:	Centre Scientifique de Monaco (CSM), Government of the Principality of Monaco (GPM)
Date Deposited:	18 Aug 2016 05:05
FoR Codes:	06 BIOLOGICAL SCIENCES > 0604 Genetics > 060405 Gene Expression (incl Microarray and other genome-wide approaches) @ 100%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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