Synthetic B-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine

Ramanathan, Babu, Poh, Chit Laa, Kirk, Kristin, McBride, William John Hannan, Aaskov, John, and Grollo, Lara (2016) Synthetic B-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine. PLoS ONE, 11 (5). e0155900. pp. 1-22.

[img]
Preview
PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (2MB) | Preview
View at Publisher Website: http://dx.doi.org/10.1371/journal.pone.0...
 
5
16


Abstract

Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.

Item ID: 44693
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

© 2016 Ramanathan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

https://creativecommons.org/licenses/by/4.0/

Funders: Swinburne University of Technology (SUT), International Postgraduate Research Scholarship (IPRS), Sunway University (SU) Kuala Lumpur, Malaysia
Projects and Grants: SUT Researcher Development Scheme, SUT Grollo Ruzzene Foundation Research Grant., SU Research Fellowship, SU INT-VCO-2016-01
Date Deposited: 21 Jul 2016 02:50
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060506 Virology @ 34%
11 MEDICAL AND HEALTH SCIENCES > 1103 Clinical Sciences > 110309 Infectious Diseases @ 33%
11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110702 Applied Immunology (incl Antibody Engineering, Xenotransplantation and T-cell Therapies) @ 33%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
Downloads: Total: 16
Last 12 Months: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page