Synthetic B-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine
Ramanathan, Babu, Poh, Chit Laa, Kirk, Kristin, McBride, William John Hannan, Aaskov, John, and Grollo, Lara (2016) Synthetic B-cell epitopes eliciting cross-neutralizing antibodies: strategies for future dengue vaccine. PLoS ONE, 11 (5). e0155900. pp. 1-22.
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Abstract
Dengue virus (DENV) is a major public health threat worldwide. A key element in protection from dengue fever is the neutralising antibody response. Anti-dengue IgG purified from DENV-2 infected human sera showed reactivity against several peptides when evaluated by ELISA and epitope extraction techniques. A multi-step computational approach predicted six antigenic regions within the E protein of DENV-2 that concur with the 6 epitopes identified by the combined ELISA and epitope extraction approach. The selected peptides representing B-cell epitopes were attached to a known dengue T-helper epitope and evaluated for their vaccine potency. Immunization of mice revealed two novel synthetic vaccine constructs that elicited good humoral immune responses and produced cross-reactive neutralising antibodies against DENV-1, 2 and 3. The findings indicate new directions for epitope mapping and contribute towards the future development of multi-epitope based synthetic peptide vaccine.
| Item ID: | 44693 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1932-6203 |
| Additional Information: | © 2016 Ramanathan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. https://creativecommons.org/licenses/by/4.0/ |
| Funders: | Swinburne University of Technology (SUT), International Postgraduate Research Scholarship (IPRS), Sunway University (SU) Kuala Lumpur, Malaysia |
| Projects and Grants: | SUT Researcher Development Scheme, SUT Grollo Ruzzene Foundation Research Grant., SU Research Fellowship, SU INT-VCO-2016-01 |
| Date Deposited: | 21 Jul 2016 02:50 |
| FoR Codes: | 31 BIOLOGICAL SCIENCES > 3107 Microbiology > 310706 Virology @ 34% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3202 Clinical sciences > 320211 Infectious diseases @ 33% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3204 Immunology > 320402 Applied immunology (incl. antibody engineering, xenotransplantation and t-cell therapies) @ 33% |
| SEO Codes: | 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100% |
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