Stewart, Michael James, Stewart, Praphaporn, and Rivera-Posada, Jairo (2015) De novo assembly of the transcriptome of Acanthaster plancitestes. Molecular Ecology Resources, 15 (4). pp. 953-966.

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Abstract

A key strategy to reduce coral loss is the development of effective control method for the corallivorous crown-of-thorns sea star (Acanthaster planci), an omnipresent scourge and threat to the biodiversity of reefs in the Indo-Pacific region. Limited genetic resources are available for this highly fecund species. In this study, we explored one aspect at the heart of A. planci outbreaks, the male reproductive system. Using high-throughput sequencing technology, we report for first time the production of a comprehensive transcriptomic data set for the testes of A. placni that can aid in understanding the molecular mechanisms involved in A. planci spermatogenesis and fertilization. Through de novo transcriptome sequencing, we produced 52 965 998 raw reads corresponding to 4.76 Gb clean read data. From this, 243 870 contigs were assembled with Trinity and used to construct 92 792 unigenes. Distinct genes were then annotated with blastx yielding 30 810 unigenes above the cut-off E-value set at 10−5, with ESTScan database query analyses yielding up to 5366 unigenes to known hits. The identification of genes directly involved in sperm development (DEAD-box family proteins), motility, fertilization and signalling (Bindin/Speract receptor) are also discussed.

Item ID:	44357
Item Type:	Article (Research - C1)
ISSN:	1755-0998
Keywords:	bindin; crown-of-thorns sea star; illumina sequencing; spermatogenesis; testis
Funders:	Australian Institute of Marine Science (AIMS), ARC Centre of Excellence for Coral Reef Studies, John and Laurie Proud 2013 Fellowship
Date Deposited:	17 Jun 2016 01:47
FoR Codes:	05 ENVIRONMENTAL SCIENCES > 0501 Ecological Applications > 050103 Invasive Species Ecology @ 30% 06 BIOLOGICAL SCIENCES > 0608 Zoology > 060803 Animal Developmental and Reproductive Biology @ 40% 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060205 Marine and Estuarine Ecology (incl Marine Ichthyology) @ 30%
SEO Codes:	96 ENVIRONMENT > 9604 Control of Pests, Diseases and Exotic Species > 960407 Control of Pests, Diseases and Exotic Species in Marine Environments @ 50% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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