Resistance against Echinostoma caproni (Trematoda) secondary infections in mice is not dependent on the ileal protein production

Cortés, Alba, Sotillo, Javier, Muñoz-Antolí, Carla, Martín-Grau, Carla, Esteban, J. Guillermo, and Toledo, Rafael (2016) Resistance against Echinostoma caproni (Trematoda) secondary infections in mice is not dependent on the ileal protein production. Journal of Proteomics, 140. pp. 37-47.

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Abstract

Echinostoma caproni (Trematoda: Echinostomatidae) is an intestinal trematode, which has been widely employed to investigate the factors determining the rejection of intestinal helminths. Protein production patterns of intestinal epithelial cells are related to the infection-induced changes that determine the course of E. caproni infections. Herein, we compare the protein production profiles in the ileum of four experimental groups of mice: control; infected; dewormed and reinfected. Worm burdens were significantly lower in secondary infections, confirming the generation of partial resistance to homologous secondary infections in mice. However, quantitative comparison by 2D-DIGE showed that the protein production profile is similar in control and dewormed mice, and after primary and secondary E. caproni infections. These results showed that, unexpectedly, protein production changes in E. caproni infections are not responsible of resistance development. Fifty-one protein spots were differentially produced between control/treated and infected/reinfected mice and 37 of them were identified by mass spectrometry. The analysis of differentially abundant proteins indicate that cell metabolism and the regulation of proliferation and cell death are the most affected processes after primary and secondary E. caproni infections. These results provide new insights into the proteins involved in the regulation of tissue homeostasis after intestinal infection.

Item ID: 43699
Item Type: Article (Research - C1)
ISSN: 1876-7737
Keywords: Echinostoma caproni; infection resistance; mouse intestine; praziquantel; 2D-DIGE
Funders: Ministerio de Economía y Competitividad (MECD), Conselleria d'Educació, Generalitat Valenciana (CE), Red de Investigación Cooperativa en Enfermedades Tropicales (RICET), Subdirección General de Redes y Centros de Investigación Cooperativa (SGRCIC), Ministerio de Sanidad y Consumo (MSC)
Projects and Grants: MECD BFU2013-41040-P, CE PROMETEO2014-083 Fase II, RICET RD12/0018/0013, SGRCIC IV National Program of I+D+I 2008 – 2011, ISCIII
Date Deposited: 26 Jul 2016 03:56
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 10%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 70%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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