Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni

Cortés, Alba, Sotillo-Gallego, Javier, Muñoz-Antolí, Carla, Trelis, Maria, Esteban, J. Guillermo, and Toledo, Rafael (2016) Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni. Parasites & Vectors, 9. 185. pp. 1-10.

[img]
Preview
PDF (Published Version) - Published Version
Available under License Creative Commons Attribution.

Download (843kB) | Preview
View at Publisher Website: http://dx.doi.org/10.1186/s13071-016-146...
 
4
34


Abstract

Background: Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments.

Methods: To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed.

Results: A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host.

Conclusions: The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival.

Item ID: 43698
Item Type: Article (Research - C1)
ISSN: 1756-3305
Keywords: Echinostoma caproni, helminth, excretory/secretory products, proteome plasticity, 2-dimensional gel electrophoresis
Additional Information:

© 2016 Cortés et al. Open Access

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Funders: Minesterio de Economía y Competitividad (MEC) Spain, Conselleria d’Educació, Generalitat Valenciana (CEGV) Spain, Red de Investigación Cooperativa en Enfermedades Tropicales (RICET)
Projects and Grants: MEC BFU2013-41040-P, CEGV PROMETEO2014-083 Fase II, RICET RD12/0018/0013
Date Deposited: 22 Jul 2016 01:35
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 10%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 70%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
Downloads: Total: 34
Last 12 Months: 18
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page