Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni
Cortés, Alba, Sotillo-Gallego, Javier, Muñoz-Antolí, Carla, Trelis, Maria, Esteban, J. Guillermo, and Toledo, Rafael (2016) Definitive host influences the proteomic profile of excretory/secretory products of the trematode Echinostoma caproni. Parasites & Vectors, 9. 185. pp. 1-10.
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Abstract
Background: Echinostoma caproni is an intestinal trematode extensively used as experimental model for the study of factors that determine the course of intestinal helminth infections, since this markedly depends on the host species. Although the host-dependent mechanisms for either chronic establishment or early parasite rejection have been broadly studied, little is known regarding the parasite response against different host environments.
Methods: To identify host-dependent differentially expressed proteins, a comparative proteomic analysis of the excretory/secretory products released from E. caproni adults, isolated from hosts displaying different compatibility with this trematode, was performed.
Results: A total of 19 differential protein spots were identified (14 overexpressed in mice and 5 overexpressed in rats). The establishment of chronic infections in mice is mainly associated with the overexpression by adult worms of antioxidant and detoxifying enzymes (e.g. glutathione S-transferase, hydroxyacylglutathione hydrolase, thiopurine S-transferase, etc.) and metabolic enzymes like enolase, leucine aminopeptidase or malate dehydrogenase. However, the overexpression of cathepsin L and the structural protein actin observed in worms isolated from rats seems not to be effective for the colonization of the intestinal mucosa of this host.
Conclusions: The observed differences suggest that protein expression and/or release is modulated by the local environment generated inside the host and provide useful insights in regards to the resistance mechanisms developed by parasites to ensure their long-term survival.
| Item ID: | 43698 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1756-3305 |
| Keywords: | Echinostoma caproni, helminth, excretory/secretory products, proteome plasticity, 2-dimensional gel electrophoresis |
| Additional Information: | © 2016 Cortés et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| Funders: | Minesterio de Economía y Competitividad (MEC) Spain, Conselleria d’Educació, Generalitat Valenciana (CEGV) Spain, Red de Investigación Cooperativa en Enfermedades Tropicales (RICET) |
| Projects and Grants: | MEC BFU2013-41040-P, CEGV PROMETEO2014-083 Fase II, RICET RD12/0018/0013 |
| Date Deposited: | 22 Jul 2016 01:35 |
| FoR Codes: | 31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310407 Host-parasite interactions @ 20% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 10% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics) @ 70% |
| SEO Codes: | 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50% |
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