Proteomic characterization of the internalization of Opisthorchis viverrini excretory/secretory products in human cells

Chaiyadet, Sujittra, Smout, Michael, Laha, Thewarach, Sripa, Banchob, Loukas, Alex, and Sotillo, Javier (2017) Proteomic characterization of the internalization of Opisthorchis viverrini excretory/secretory products in human cells. Parasitology International, 66 (4). pp. 494-502.

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Abstract

The association between liver fluke infection caused by Opisthorchis viverrini and cholangiocarcinoma (CCA — hepatic cancer of the bile duct epithelium) has been well established. Multiple mechanisms play a role in the development of CCA, but the excretory/secretory products released by O. viverrini (OvES) represent the major interface between the parasite and its host, and their uptake by biliary epithelial cells has been suggested to be responsible for proliferation of cholangiocytes, the cells that line the biliary epithelium. Despite recent progress in the study of the molecular basis of O. viverrini–host interactions, little is known about the effects that OvES induces upon internalization by host cells. In the present study we incubated non-cancerous human cholangiocytes (H69) and human colon cancer (CaCo-2) cells with OvES and performed a time-course quantitative proteomic analysis on the cells to determine the early changes induced by the parasite. Different KEGG pathways were altered in H69 cells compared to Caco-2 cells: glycolysis/gluconeogenesis and protein processing in the endoplasmic reticulum. In addition, the Reactome pathway analysis showed a predominance of proteins involved in cellular pathways related to apoptosis and apoptotic execution phase in H69 cells after incubation with OvES. The present study provides the first proteomic analysis to address the molecular mechanisms by which OvES products interact with host cells, and Sheds light on the cellular processes involved in O. viverrini-induced CCA.

Item ID: 43697
Item Type: Article (Research - C1)
ISSN: 1873-0329
Keywords: Opisthorchis viverrini; excretory/secretory products; cholangiocytes; caco-2 cells; proteomics; iTRAQ
Funders: National Health and Medical Research Council of Australia (NHMRC), National Institute of Allergy and Infectious Disease (NIAID), National Institutes of Health (NIH), USA, Thailand Research Fund, TRF
Projects and Grants: NHMRC Project 613669, NHMRC Project 1037304, NIAID Tropical Medicine Research Collaboration Grant, NIH P50AI098639, TRF RTA 5680006, TRF Grant No. PHD/0205/2551
Date Deposited: 13 Sep 2016 03:05
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 10%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 70%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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