Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish

Andrewartha, Sarah J., Munns, Suzanne L., and Edwards, Ashley (2016) Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish. Conservation Physiology, 4 (1). cow006. pp. 1-6.

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Haemoglobin concentration ([Hb]) is measured for a wide variety of animal studies. The use of point-of-care devices, such as the HemoCue, is becoming increasingly common because of their portability, relative ease of use and low cost. In this study, we aimed to determine whether the [Hb] of blue-tongued skink (Tiliqua nigrolutea) blood can be determined accurately using the HemoCue and whether the HemoCue overestimates the [Hb] of reptile blood in a similar manner to fish blood. Additionally, we aimed to test whether ploidy affected [Hb] determined by the HemoCue using blood from diploid and triploid Atlantic salmon (Salmo salar). The HemoCue Hb 201⁺ systematically overestimated [Hb] in both blue-tongued skinks and Atlantic salmon, and there was no difference between calibration equations determined for diploid or triploid salmon. The overestimation was systematic in both species and, as such, [Hb] determined by the HemoCue can be corrected using appropriate calibration equations.

Item ID: 43423
Item Type: Article (Research - C1)
ISSN: 2051-1434
Keywords: diplod, fish, HemoCue, haemoglobin, reptile, blood, triploid
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© The Author 2016. Published by Oxford University Press and the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Funders: R. Lobb Endowment (RLE), Commonwealth Scientific and Industrial Research Organisation (CSIRO), Salmon Enterprises of Tasmania (SET)
Date Deposited: 29 Jul 2016 04:45
FoR Codes: 31 BIOLOGICAL SCIENCES > 3109 Zoology > 310912 Comparative physiology @ 20%
31 BIOLOGICAL SCIENCES > 3109 Zoology > 310999 Zoology not elsewhere classified @ 35%
32 BIOMEDICAL AND CLINICAL SCIENCES > 3201 Cardiovascular medicine and haematology > 320102 Haematology @ 45%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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