Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata

Snyder, S., Nadler, L. E., Bayley, J. S., Svendsen, M. B. S., Johansen, J. L., Domenici, P., and Steffensen, J. F. (2016) Effect of closed v. intermittent-flow respirometry on hypoxia tolerance in the shiner perch Cymatogaster aggregata. Journal of Fish Biology, 88 (1). pp. 252-264.

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Abstract

This study compares the critical oxygen saturation (O-2crit) levels of the shiner perch Cymatogaster aggregata obtained using two different methods wherein hypoxia is induced either by the fish's respiration (closed respirometry) or by degassing oxygen with nitrogen (intermittent-flow respirometry). Fish exhibited loss of equilibrium at a higher O-2 saturation in the closed respirometry method when compared with the intermittent-flow method. Utilization of closed respirometry yielded O-2crit measurements that were almost twice as high as those obtained with intermittent-flow respirometry. The lower hypoxia tolerance in closed respirometry is consistent with additional stress, caused by a build-up of ammonia and carbon dioxide and a faster rate in dissolved oxygen decline. The results indicate that these two methods of determining hypoxia tolerance in aquatic organisms are not comparable, and that much care should be given to method choice.

Item ID: 43224
Item Type: Article (Research - C1)
ISSN: 0022-1112
Keywords: aquatic breathers, critical oxygen saturation level, hypoxic, physiology, respiration, stress
Funders: Ragen Fellowship, Australian Postgraduate Award (APA), International Postgraduate Research Scholarship (IPRS), National Science Foundation (NSF), Scripps Institution of Oceanography
Projects and Grants: NSF DGE-1144086
Date Deposited: 24 Feb 2016 07:35
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070405 Fish Physiology and Genetics @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8301 Fisheries - Aquaculture > 830102 Aquaculture Fin Fish (excl. Tuna) @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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