Influence of nutrients on oxidation of low level methane by mixed methanotrophic consortia

Karthikeyan, Obulisamy Parthiba, Chidambarampadmavathy, Karthigeyan, Nadarjan, Saravanan, and Heimann, Kirsten (2016) Influence of nutrients on oxidation of low level methane by mixed methanotrophic consortia. Environmental Science and Pollution Research, 23 (5). pp. 4346-4357.

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Abstract

Low-level methane emissions from coal mine ventilation air (CMV-CH4; i.e., 1 % CH4) can significantly contribute to global climate change, and therefore, treatment is important to reduce impacts. To investigate CMV-CH4 abatement potential, five different mixed methanotrohic consortia (MMCs) were established from soil/sediment sources, i.e., landfill top cover soil, bio-solid compost, vegetated humus soil, estuarine and marine sediments. Enrichment conditions for MMCs were as follows: nitrate mineral salt (NMS) medium, pH ~ 6.8; 25 °C; 20–25 % CH4; agitation 200 rpm; and culture period 20 days, in mini-bench-top bioreactors. The enriched cultures were supplemented with extra carbon (methanol 0.5–1.5 %, formate 5–15 mM, and acetate 5–15 mM), nitrogen (nitrate 0.5–1.5 g L−1, ammonium 0.1–0.5 g L−1, or urea: 0.1–0.5 g L−1), and trace elements (copper 1–5 μM, iron 1–5 μM, and zinc 1–5 μM) in different batch experiments to improve low-level CH4 abatement. Average CH4 oxidation capacities (MOCs) of MMCs varied between 1.712 ± 0.032 and 1.963 ± 0.057 mg g−1DWbiomass h−1. Addition of formate improved the MOCs of MMCs, but the dose-response varied for different MMCs. Acetate, nitrate and copper had no significant effect on MOCs, while addition of methanol, ammonium, urea, iron and zinc impacted negatively. Overall, MMCs enriched from marine sediments and landfill top cover soil showed high MOCs which were largely resilient to nutrient supplementation, suggesting a strong potential for biofilter development for industrial low-level CH4 abatement, such as those present in CMV.

Item ID: 43079
Item Type: Article (Refereed Research - C1)
Keywords: global warming; coal mine; methane; methanotrophs; soil/sediments; nutrients; methanol; metals
ISSN: 1614-7499
Funders: Advanced Manufacturing Co-operative Research Centre (AMCRC)
Projects and Grants: AMCRC 2.3.4
Date Deposited: 26 May 2016 00:04
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070401 Aquaculture @ 30%
09 ENGINEERING > 0907 Environmental Engineering > 090703 Environmental Technologies @ 30%
10 TECHNOLOGY > 1003 Industrial Biotechnology > 100305 Industrial Microbiology (incl Biofeedstocks) @ 40%
SEO Codes: 84 MINERAL RESOURCES (excl. Energy Resources) > 8498 Environmentally Sustainable Mineral Resource Activities > 849802 Management of Greenhouse Gas Emissions from Mineral Resource Activities @ 100%
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