A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences

Li, Juan, Chen, Fen, Sugiyama, Hiromu, Blair, David, Lin, Rui-Qing, and Zhu, Xing-Quan (2015) A specific indel marker for the Philippines Schistosoma japonicum revealed by analysis of mitochondrial genome sequences. Parasitology Research, 114 (7). pp. 2697-2704.

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Abstract

In the present study, near-complete mitochondrial (mt) genome sequences for Schistosoma japonicum from different regions in the Philippines and Japan were amplified and sequenced. Comparisons among S. japonicum from the Philippines, Japan, and China revealed a geographically based length difference in mt genomes, but the mt genomic organization and gene arrangement were the same. Sequence differences among samples from the Philippines and all samples from the three endemic areas were 0.57-2.12 and 0.76-3.85 %, respectively. The most variable part of the mt genome was the non-coding region. In the coding portion of the genome, protein-coding genes varied more than rRNA genes and tRNAs. The near-complete mt genome sequences for Philippine specimens were identical in length (14,091 bp) which was 4 bp longer than those of S. japonicum samples from Japan and China. This indel provides a unique genetic marker for S. japonicum samples from the Philippines. Phylogenetic analyses based on the concatenated amino acids of 12 protein-coding genes showed that samples of S. japonicum clustered according to their geographical origins. The identified mitochondrial indel marker will be useful for tracing the source of S. japonicum infection in humans and animals in Southeast Asia.

Item ID: 41825
Item Type: Article (Research - C1)
ISSN: 1432-1955
Keywords: Schistosoma japonicum, schistosomiasis, mitochondrial (mt) genome, the Philippines, indel marker, comparative analyses, phylogeny
Funders: Pearl River Science and Technology (PRST), Guangdong Academy of Agricultural Sciences (GAAS), Science Fund for Creative Research Groups of Gansu Province (SFCRGG), Special Fund for Agro-Scientific Research in the Public Interest (SFASR)
Projects and Grants: PRST New Star Project Grant No. 2014 J2200096, GAAS Grant No. 201413, SFCRGG Grant No. 120RJIA006, SFASR Grant No. 201303037
Date Deposited: 08 Dec 2015 18:41
FoR Codes: 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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