Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates

Sotillo, Javier, Pearson, Mark, Potriquet, Jeremy, Becker, Luke, Pickering, Darren, Mulvenna, Jason, and Loukas, Alex (2016) Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates. International Journal for Parasitology, 46 (1). pp. 1-5.

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Abstract

Herein we show for the first time that Schistosoma mansoni adult worms secrete exosome-like extracellular vesicles ranging from 50 to 130 nm in size. Extracellular vesicles were collected from the excretory/secretory products of cultured adult flukes and purified by Optiprep density gradient, resulting in highly pure extracellular vesicle preparations as confirmed by transmission electron microscopy and Nanosight tracking analysis. Extracellular vesicle proteomic analysis showed numerous known vaccine candidates, potential virulence factors and molecules implicated in feeding. These findings provide new avenues for the exploration of host–schistosome interactions and offer a potential mechanism by which some vaccine antigens exert their protective efficacy.

Item ID: 41072
Item Type: Article (Research - C1)
ISSN: 1879-0135
Keywords: Schistosoma mansoni; extracellular vesicles; exosomes; proteomics; vaccine
Funders: National Health and Medical Research Council of Australia (NHMRC)
Date Deposited: 02 Dec 2015 01:24
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 20%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 10%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 70%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
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