Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates
Sotillo, Javier, Pearson, Mark, Potriquet, Jeremy, Becker, Luke, Pickering, Darren, Mulvenna, Jason, and Loukas, Alex (2016) Extracellular vesicles secreted by Schistosoma mansoni contain protein vaccine candidates. International Journal for Parasitology, 46 (1). pp. 1-5.
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Abstract
Herein we show for the first time that Schistosoma mansoni adult worms secrete exosome-like extracellular vesicles ranging from 50 to 130 nm in size. Extracellular vesicles were collected from the excretory/secretory products of cultured adult flukes and purified by Optiprep density gradient, resulting in highly pure extracellular vesicle preparations as confirmed by transmission electron microscopy and Nanosight tracking analysis. Extracellular vesicle proteomic analysis showed numerous known vaccine candidates, potential virulence factors and molecules implicated in feeding. These findings provide new avenues for the exploration of host–schistosome interactions and offer a potential mechanism by which some vaccine antigens exert their protective efficacy.
| Item ID: | 41072 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 1879-0135 |
| Keywords: | Schistosoma mansoni; extracellular vesicles; exosomes; proteomics; vaccine |
| Funders: | National Health and Medical Research Council of Australia (NHMRC) |
| Date Deposited: | 02 Dec 2015 01:24 |
| FoR Codes: | 31 BIOLOGICAL SCIENCES > 3104 Evolutionary biology > 310407 Host-parasite interactions @ 20% 32 BIOMEDICAL AND CLINICAL SCIENCES > 3207 Medical microbiology > 320704 Medical parasitology @ 10% 31 BIOLOGICAL SCIENCES > 3101 Biochemistry and cell biology > 310109 Proteomics and intermolecular interactions (excl. medical proteomics) @ 70% |
| SEO Codes: | 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50% 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50% |
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