Lack of a functioning P2X7 receptor leads to increased susceptibility to toxoplasmic ileitis

Miller, Catherine M., Zakrzewski, Alana M., Robinson, Dionne P., Fuller, Stephen J., Walker, Robert A., Ikin, Rowan J., Bao, Shisan J., Grigg, Michael E., Wiley, James S., and Smith, Nicholas C. (2015) Lack of a functioning P2X7 receptor leads to increased susceptibility to toxoplasmic ileitis. PLoS ONE, 10 (6). e0129048. pp. 1-17.

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Abstract

Background: Oral infection of C57BL/6J mice with the protozoan parasite Toxoplasma gondii leads to a lethal inflammatory ileitis.

Principal Findings: Mice lacking the purinergic receptor P2X7R are acutely susceptible to toxoplasmic ileitis, losing significantly more weight than C57BL/6J mice and exhibiting much greater intestinal inflammatory pathology in response to infection with only 10 cysts of T. gondii. This suscep-tibility is not dependent on the ability of P2X7R-deficient mice to control the parasite, which they accomplish just as efficiently as C57BL/6J mice. Rather, susceptibility is associated with elevated ileal concentrations of pro-inflammatory cytokines, reactive nitrogen interme-diates and altered regulation of elements of NFκB activation in P2X7R-deficient mice.

Conclusions: Our data support the thesis that P2X7R, a well-documented activator of pro-inflammatory cytokine production, also plays an important role in the regulation of intestinal inflammation.

Item ID: 39201
Item Type: Article (Research - C1)
ISSN: 1932-6203
Additional Information:

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication

Funders: Australian Research Council (ARC), National Institute of Health (NIAID), Canadian Institute for Advanced Research (CIFAR)
Projects and Grants: ARC Discovery grant DP0666515
Date Deposited: 17 Jun 2015 01:46
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1107 Immunology > 110707 Innate Immunity @ 50%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 30%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 20%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 60%
92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 40%
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