A quantitative proteomic analysis of the tegumental proteins from Schistosoma mansoni schistosomula reveals novel potential therapeutic targets

Sotillo, Javier, Pearson, Mark, Becker, Luke, Mulvenna, Jason, and Loukas, Alex (2015) A quantitative proteomic analysis of the tegumental proteins from Schistosoma mansoni schistosomula reveals novel potential therapeutic targets. International Journal for Parasitology, 45 (8). pp. 505-516.

[img]
Preview
PDF (Published Version) - Published Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (2MB) | Preview
View at Publisher Website: http://dx.doi.org/10.1016/j.ijpara.2015....
 
35
44


Abstract

The tegument of Schistosoma mansoni plays an integral role in host–parasite interactions, particularly during the transition from the free-living cercariae to the intra-mammalian schistosomula stages. This developmental period is characterised by the transition from a trilaminate surface to a heptalaminate tegument that plays key roles in immune evasion, nutrition and excretion. Proteins exposed at the surface membranes of newly transformed schistosomula are therefore thought to be prime targets for the development of new vaccines and drugs for schistosomiasis. Using a combination of tegumental labelling and high-throughput quantitative proteomics, more than 450 proteins were identified on the apical membrane of S. mansoni schistosomula, of which 200 had significantly regulated expression profiles at different stages of schistosomula development in vitro, including glucose transporters, sterols, heat shock proteins, antioxidant enzymes and peptidases. Current vaccine antigens were identified on the apical membrane (Sm-TSP-1, calpain) or sub-tegumental (Sm-TSP-2, Sm29) fractions of the schistosomula, displaying localisation patterns that, in some cases, differ from that in the adult stage fluke. This work provides the first known in-depth proteomic analysis of the surface-exposed proteins in the schistosomula tegument, and some of the proteins identified are clear targets for the generation of new vaccines and drugs against schistosomiasis.

Item ID: 38949
Item Type: Article (Research - C1)
ISSN: 1879-0135
Keywords: Schistosoma mansoni, Schistosomula, iTRAQ, proteomics, tegument
Additional Information:

© 2015 The Authors. Published by Elsevier Ltd. on behalf of Autralian Society for Parasitology Inc. This is an open access article under the CC BY-NC-ND licence.

Funders: ational Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), USA, National Health and Medical Research Council of Australia (NHMRC)
Date Deposited: 14 Sep 2015 01:46
FoR Codes: 06 BIOLOGICAL SCIENCES > 0603 Evolutionary Biology > 060307 Host-Parasite Interactions @ 20%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics) @ 70%
11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 10%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 50%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
Downloads: Total: 44
Last 12 Months: 1
More Statistics

Actions (Repository Staff Only)

Item Control Page Item Control Page