Development of multiplex microsatellite PCR panels for the seagrass Thalassia hemprichii (Hydrocharitaceae)
Van Dijk, Kor-Jent, Mellors, Jane, and Waycott, Michelle (2014) Development of multiplex microsatellite PCR panels for the seagrass Thalassia hemprichii (Hydrocharitaceae). Applications in Plant Sciences, 2 (11). 1400078. pp. 1-5.
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Abstract
Premise of the study: New microsatellites were developed for the seagrass Thalassia hemprichii (Hydrocharitaceae), a long-lived seagrass species that is found throughout the shallow waters of tropical and subtropical Indo-West Pacific. Three multiplex PCR panels were designed utilizing new and previously developed markers, resulting in a toolkit for generating a 16-locus genotype.
Methods and Results: Through the use of microsatellite enrichment and next-generation sequencing, 16 new, validated, polymorphic microsatellite markers were isolated. Diversity was between two and four alleles per locus totaling 36 alleles. These markers, plus previously developed microsatellite markers for T. hemprichii and T. testudinum, were tested for suitability in multiplex PCR panels.
Conclusions: The generation of an easily replicated suite of multiplex panels of codominant molecular markers will allow for high-resolution and detailed genetic structure analysis and clonality assessment with minimal genotyping costs. We suggest the establishment of a T. hemprichii primer convention for the unifi cation of future data sets.
| Item ID: | 38396 |
|---|---|
| Item Type: | Article (Research - C1) |
| ISSN: | 2168-0450 |
| Keywords: | clonality; Hydrocharitaceae; microsatellites; population genetics; seagrass; Thalassia hemprichii |
| Additional Information: | © 2014 van Dijk et al. Published by the Botanical Society of America. This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
| Date Deposited: | 23 Apr 2015 01:48 |
| FoR Codes: | 06 BIOLOGICAL SCIENCES > 0604 Genetics > 060411 Population, Ecological and Evolutionary Genetics @ 75% 06 BIOLOGICAL SCIENCES > 0602 Ecology > 060207 Population Ecology @ 25% |
| SEO Codes: | 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50% 97 EXPANDING KNOWLEDGE > 970105 Expanding Knowledge in the Environmental Sciences @ 50% |
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