Development of genome-wide microsatellite genetic resources in a commercially important African freshwater fish species – the Nile perch, Lates niloticus

Basiita, Rose K., Zenger, Kyall R., Mwanja, Matthew T., and Jerry, Dean R. (2015) Development of genome-wide microsatellite genetic resources in a commercially important African freshwater fish species – the Nile perch, Lates niloticus. Animal Genetics, 46 (3). p. 340.

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Abstract

[Extract] Nile perch, Lates niloticus, is a commercially important African freshwater species with limited available genetic resources. This study provides 24 polymorphic microsatellite markers for one of the most commercially important fisheries to the African continent validated in L. niloticus. Next-generation sequencing on Roche 454 GS-FLX instrumentation yielded ~122 000 sequences, which have been deposited at the NCBI (Accession no. SRP051308), and allowed the design of primers for 285 in silico perfect microsatellite loci (Table S1). Thirty-one microsatellite loci were tested for polymorphisms. Additionally, 16 microsatellites previously characterised for Lates calcarifer (a sister species) were tested in L. niloticus.

Item ID: 37736
Item Type: Article (Short Note)
ISSN: 1365-2052
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A version of this publication was included as Chapter 2 of the following PhD thesis: Basiita, Rose Komugisha (2016) Genetic structure and biogeography of three commercially important African freshwater fishes: Lates niloticus, Bagrus docmak and Bagrus bayad. PhD thesis, James Cook University, which is available Open Access in ResearchOnline@JCU. Please see the Related URLs for access.

Funders: AusAID, James Cook University
Date Deposited: 03 Jun 2015 22:56
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0704 Fisheries Sciences > 070405 Fish Physiology and Genetics @ 100%
SEO Codes: 83 ANIMAL PRODUCTION AND ANIMAL PRIMARY PRODUCTS > 8302 Fisheries - Wild Caught > 830204 Wild Caught Fin Fish (excl. Tuna) @ 100%
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