Seixas, Fabiana Kömmling, Silva , Éverton Fagonde, Hartwig, Daiane Drawanz, Cerqueira, Gustavo Maia, Amaral, Marta, Fagundes, Michel Quevedo, Dossa, Robson Grando, and Dellagostin, Odir Antônio (2007) Recombinant Mycobacterium bovis BCG expressing the LipL32 antigen of Leptospira interrogans protects hamsters from challenge. Vaccine, 26 (1). pp. 88-95.

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Abstract

The bacillus Calmette--Guerin (BCG), a live attenuated Mycobacterium bovis strain is considered a promising candidate as a vector system for delivery of foreign antigens to the immune system. The gene coding for the Leptospira interrogans external membrane protein LipL32, a highly immunogenic antigen found in all pathogenic leptospira, was cloned into several mycobacterial vectors for expression in BCG. Hamsters immunized with recombinant BCG (rBCG) expressing LipL32 were protected against mortality (P≤0.05) upon challenge with a lethal inoculum of L. interrogans serovar Copenhageni. Autopsy examination did not reveal macroscopic or histological evidence of disease in rBCG immunized hamsters that survived lethal challenge. The data presented here further enhance the status of LipL32 as a promising candidate antigen for use in the control of leptospirosis, when presented to the immune system by an appropriate delivery system.

Item ID:	37430
Item Type:	Article (Research - C1)
ISSN:	1873-2518
Keywords:	leptospirosis; recombinant BCG; LipL32
Funders:	Brazilian National Council for Scientific and Technological Development (CNPq), Bio-Manguinhos, Oswaldo Cruz Foundation (OCF), Brazilian Ministry of Health (BMH), Fogarty International Center (FIC), CAPES
Projects and Grants:	CNPq grant 473006/2006-5, FIC grant TW00919
Date Deposited:	26 Mar 2015 03:44
FoR Codes:	06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 50% 10 TECHNOLOGY > 1004 Medical Biotechnology > 100499 Medical Biotechnology not elsewhere classified @ 50%
SEO Codes:	97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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