Characterization of the immunogenic and antigenic potential of putative lipoproteins from Leptospira interrogans

Hartwig, Daiane D., Seixas, Fabiana K., Cerqueira, Gustavo M., McBride, Alan J.A., and Dellagostin, Odir A. (2011) Characterization of the immunogenic and antigenic potential of putative lipoproteins from Leptospira interrogans. Current Microbiology, 62 (4). pp. 1337-1341.

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Abstract

The search for a vaccine capable of conferring heterologous protection, through the identification of conserved and cross-protective antigens, remains an ongoing priority in leptospirosis research. In the present study, an in silico analysis was used to identify potentially protective lipoproteins from Leptospira interrogans serovar Copenhageni. Eight putative lipoproteins were selected (LIC10009, LIC10054, LIC10091, LIC11058, LIC11567, LIC13059, LIC13305, and LIC20172), cloned and expressed in Escherichia coli and purified by affinity chromatography. The recombinant proteins were used to inoculate mice and the subsequent humoral immune response was evaluated by ELISA. Seven of the potential lipoproteins induced a significant IgG response. Furthermore, all of the recombinant proteins were recognized by antibodies present in the sera of severe leptospirosis patients. These putative lipoproteins exhibited potential for further evaluation as prospective vaccine candidates.

Item ID: 37415
Item Type: Article (Research - C1)
ISSN: 1432-0991
Funders: Brazilian National Research Foundation (CNPq), Research Support Foundation for the State of Bahia (FAPESB)
Projects and Grants: CNPq grant 475540/2008-5, FAPESB grant PES-0092/2008
Date Deposited: 11 Mar 2015 03:37
FoR Codes: 06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060502 Infectious Agents @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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