Effect of excretory/secretory products of abomasal parasites on epithelial tight junctions

Walker, L.R., Simpson, H.V., Simcock, D.C., and Pedley, K.C. (2009) Effect of excretory/secretory products of abomasal parasites on epithelial tight junctions. New Zealand Journal of Zoology, 36 (4). pp. 480-481.

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Abstract

The presence of abomasal parasites is thought to be associated with an increase in the permeability of the gastric epithelium. Epithelial permeability is regulated by junctional complexes between adjacent cells. The most apical component of this junctional complex is the tight junction which functions as a paracellular diffusion barrier. Any disruption of tight junctions results in impaired barrier function and an associated increase in epithelial permeability. To investigate the effect of abomasal parasites on the integrity and barrier function of epithelia, Caco-2 cell monolayers were exposed to the excretory/ secretory products (ES) of adult Ostertagia (Teladorsagia) circumcincta and Haemonchus contortus. Changes in epithelial barrier function were monitored by measuring transepithelial electrical resistance (TEER) and tight junction integrity was visualised using immunofluorescence localisation of the tight junction-associated proteins, occludin and zonula occludens-1 (ZO-1), by confocal microsopy. Under control conditions, occludin and ZO-1 were localised to a continuous pericellular ring around individual cells when viewed from the apical surface. In cells exposed to ES for 24 h, staining of this pericellular ring was diminished in intensity and corresponded with an increase in the presence of punctuate, intracellular staining. Exposure to ES was also shown to interfere with tight junction integrity, which was detected as a decrease in TEER from 678 ± 10 Ωcm2 (control) to 526 ± 8 Ωcm2 (ES-treated) (n = 12) in 6 h. These alterations in TEER, along with intracellular changes in occludin and ZO-1 distribution, suggest that parasite ES disrupts tight junctions, leading to an increase in epithelial permeability which may be of importance in the pathology of abomasal parasitism.

Item ID: 36393
Item Type: Article (Abstract)
Additional Information:

Abstract from the 36th Conference of the New Zealand Society for Parasitology, at Massey University, Palmerston North, 23–24 October 2008.

ISSN: 1175-8821
Date Deposited: 25 Sep 2015 01:37
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060602 Animal Physiology - Cell @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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