Is the H. contortus ES product which vacuolates HeLa cells a prostaglandin or lipid?

Buering, Wiebke, Pedley, Kevin, Simcock, David, and Simpson, Heather (2009) Is the H. contortus ES product which vacuolates HeLa cells a prostaglandin or lipid? New Zealand Journal of Zoology, 36 (4). p. 486.

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Vacuolated parietal cells have been observed in tissue sections from parasitised sheep, and in vitro preparations of Haemonchus contortus excretory/ secretory (ES) products are able to induce vacuolation in HeLa and AGS cells. The mechanisms and active components have not yet been identified, although different components of ES products are being tested as possible candidates. Prostaglandins play a role in many biological processes, including host-parasite interactions, and are synthesised by both host and a number of parasites, including nematodes. Prostaglandins have been reported to be involved in vacuole formation in protozoa, but it is not certain whether prostaglandins are capable of vacuolating mammalian cells or playing a role in the pathophysiology of nematode parasitism. The aim of this study was to separate lipid components of adult H. contortus ES products and assess their possible role in vacuolation of HeLa cells. Lipids in ES products were separated by thin layer chromatography (TLC) with phosphomolybdic acid staining and commercial prostaglandin standards (PGA2, PGB2, PGD2, PGE2 and PGF2α) used as a reference. ES product lipids were additionally identified by SDSPAG E with Sudan Black staining. The prostaglandin standards were also tested on both HeLa and AGS cells cultivated on glass cover slips and the formation of vacuoles microscopically examined using Neutral Red. Lipids, but not prostaglandins, were detected by TLC and these spots were excised and tested for vacuolating activity in HeLa cells. Several lipid bands were also detected by SDS-PAG E. None of the prostaglandin standards or ES product lipids were able to induce vacuolation. These results show that it is very unlikely that the lipids in H. contortus ES products are responsible for vacuole formation in HeLa cells.

Item ID: 36392
Item Type: Article (Abstract)
ISSN: 1175-8821
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Abstract from the 36th Conference of the New Zealand Society for Parasitology, at Massey University, Palmerston North, 23–24 October 2008.

Date Deposited: 25 Sep 2015 01:41
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060602 Animal Physiology - Cell @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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