L3 and adult Ostertagia (Teladorsagia) circumcincta exhibit cyanide sensitive oxygen uptake

Simcock, D.C., Brown, S., Neale, J.D., Przemeck, S.M.C., and Simpson, H.V. (2006) L3 and adult Ostertagia (Teladorsagia) circumcincta exhibit cyanide sensitive oxygen uptake. Experimental Parasitology, 112 (1). pp. 1-7.

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Abstract

Oxygen consumption by L3 and adult Ostertagia (Teladorsagia) circumcincta was examined in vitro to determine whether oxygen can be utilised in metabolism. The oxygen concentration in the abomasal fluid of sheep infected with O. circumcincta was also measured. Rates of consumption (in nmol O2/h/1000 worms) were 13 ± 1 in sheathed L3, 34 ± 6 in exsheathed L3, and 1944 ± 495 in adult worms. Constant rates of consumption were maintained until media oxygen concentration dropped to between 10 and 20 µM. Consumption was inhibited 95% by cyanide in L3 and 74% in adults. Oxygen concentration in abomasal fluid varied between 10 and 30 µM in both infected and uninfected animals. During infection, oxygen concentration decreased slightly with increased abomasal pH, though the correlation between the two was poor (r = −0.30). In conclusion, O. circumcincta can consume oxygen and oxygen concentration at the infection site is sufficient to support at least some aerobic metabolism.

Item ID: 36379
Item Type: Article (Research - C1)
ISSN: 1090-2449
Keywords: Ostertagia circumcincta; Teladorsagia circumcincta; nematode; oxygen consumption; abomasum; aerobic metabolism; anaerobic metabolism; ATP, adenosine triphosphate; NAD+, nicotinamide adenine dinucleotide; NADH, nicotinamide adenine dinucleotide hydrogenated; Hepes, N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid;
Funders: Meat and Wool New Zealand, AGMARDT
Date Deposited: 25 Sep 2015 00:49
FoR Codes: 06 BIOLOGICAL SCIENCES > 0606 Physiology > 060604 Comparative Physiology @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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