Proteomic profile of Bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke Opisthorchis viverrini

Prasopdee, Sattrachai, Tesana, Smarn, Cantacessi, Cinzia, Laha, Thewarach, Mulvenna, Jason, Grams, Rudi, Loukas, Alex, and Sotillo, Javier (2015) Proteomic profile of Bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke Opisthorchis viverrini. Journal of Proteomics, 113. pp. 281-291.

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Abstract

The snail Bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke Opisthorchis viverrini, the major cause of cholangiocarcinoma (CCA) in Northeast Thailand. The undisputed link between CCA and O. viverrini infection has precipitated efforts to understand the molecular basis of host–parasite interactions with a view to ultimately developing new control strategies to combat this carcinogenic infection. To date most effort has focused on the interactions between the parasite and its human host, and little is known about the molecular relationships between the liver fluke and its snail intermediate host. In the present study we analyse the protein expression changes in different tissues of B. siamensis goniomphalos induced by infection with larval O. viverrini using iTRAQ labelling technology. We show that O. viverrini infection downregulates the expression of oxidoreductases and catalytic enzymes, while stress-related and motor proteins are upregulated. The present work could serve as a basis for future studies on the proteins implicated in the susceptibility/resistance of B. siamensis goniomphalos to O. viverrini, as well as studies on other pulmonate snail intermediate hosts of various parasitic flukes that infect humans.

Biological significance:

Despite the importance and high prevalence of opisthorchiasis in some regions of Southeast Asia and the direct relationship between infection by Opisthorchis viverrini and the incidence of cholangiocarcinoma, little is known of the modifications induced by this parasite in its snail intermediate hosts. This time-course study provides the first in-depth quantitative proteomic analysis of experimentally infected Bithynia siamensis goniomphalos. We show how motor and stress-related proteins are upregulated in infected snails, while O. viverrini infection downregulates the expression of oxidoreductases and catalytic enzymes. This work serves as a basis for the development of new strategies, focused on the invertebrate intermediate hosts, to control parasite transmission.

Item ID: 35934
Item Type: Article (Research - C1)
ISSN: 1876-7737
Keywords: Bithynia siamensis goniomphalos; iTRAQ; Opisthorchis; cholangiocarcinoma; intermediate host; snail-borne trematodes
Additional Information:

1874-3919/© 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).

The data set used for this paper is published as Prasopdee, Sattrachai, Tesana, Smarn, Cantacessi, Cinzia, Laha, Thewarach, Mulvenna, Jason, Grams, Rudi, Loukas, Alex, and Sotillo, Javier (2015) Data set from the proteomic analysis of Bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke Opisthorchis viverrini. Data in Brief, 2. pp. 16-20, available at http://researchonline.jcu.edu.au/38952/.

Funders: National Health and Medical Research Council of Australia (NHMRC), National Institute of Allergy and Infectious Disease, National Institutes of Health (NIH), USA, Thailand Research Fund, TRF
Projects and Grants: NHMRC Project Grant 613669, NHMRC Program Grant 1037304, NIH TMRC Grant P50AI098639, TRF Grant No. PHD/ 0027/2551
Date Deposited: 25 Jun 2015 05:24
FoR Codes: 11 MEDICAL AND HEALTH SCIENCES > 1108 Medical Microbiology > 110803 Medical Parasitology @ 100%
SEO Codes: 92 HEALTH > 9201 Clinical Health (Organs, Diseases and Abnormal Conditions) > 920109 Infectious Diseases @ 100%
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