Confidence bands for rational rate equations

Brown, Simon, and Simcock, David C. (2013) Confidence bands for rational rate equations. International Journal for Emerging Sciences, 3 (1). pp. 15-27.

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The standard Michaelis-Menten expression for the rate of an enzyme-catalysed reaction (v) can be extended using the ratio of two polynomials in the substrate concentration (s). Such rational functions are nonlinear and their behaviour can vary considerably. Comparisons of two such functions requires more than a simple consideration of a particular coefficient, especially given the coefficients do not necessarily have any mechanistic significance. A second issue is the identification of particular coefficients that contribute significantly to the overall error of the estimate of v at a given s. As the number of coefficients rises this becomes increasingly difficult. To address the first of these issues we provide a general expression for the estimated confidence band of an arbitrary rational function of this type. We employ sensitivity analysis to address the second issue and provide general expressions for the coefficient elasticities. We apply these in the analysis of the kinetics of chymotrypsin and muscle lactate dehydrogenase.

Item ID: 35821
Item Type: Article (Research - C1)
ISSN: 2222-4254
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©IJES. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Date Deposited: 20 Oct 2014 04:15
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 20%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 80%
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