Estimating enzyme kinetic parameters from apparent KMs and Vmaxs

Brown, Simon, Muhamad, Noorzaid, and Simcock, David C. (2010) Estimating enzyme kinetic parameters from apparent KMs and Vmaxs. World Academy of Science, Engineering and Technology, 4 (12). 81. pp. 431-436.

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Abstract

The kinetic properties of enzymes are often reported using the apparent KM and Vmax appropriate to the standard Michaelis-Menten enzyme. However, this model is inappropriate to enzymes that have more than one substrate or where the rate expression does not apply for other reasons. Consequently, it is desirable to have a means of estimating the appropriate kinetic parameters from the apparent values of KM and Vmax reported for each substrate. We provide a means of estimating the range within which the parameters should lie and apply the method to data for glutamate dehydrogenase from the nematode parasite of sheep Teladorsagia circumcincta.

Item ID: 35812
Item Type: Article (Research - C1)
ISSN: 2010-3778
Keywords: enzyme kinetics, glutamate dehydrogenase, interval analysis, parameter estimation
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World Academy of Science, Engineering and Technology applies the Creative Commons Attribution (CC BY) license to works we publish.

Date Deposited: 21 Oct 2014 00:56
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060107 Enzymes @ 100%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970103 Expanding Knowledge in the Chemical Sciences @ 20%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 80%
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