Teladorsagia circumcincta: survival of adults in vitro is enhanced by the presence of a mammalian cell line

Luque, A., Walker, L.R., Pedley, J.C., Pedley, K.C., Hillrichs, K., Simpson, H.V., and Simcock, D.C. (2010) Teladorsagia circumcincta: survival of adults in vitro is enhanced by the presence of a mammalian cell line. Experimental Parasitology, 124 (2). pp. 247-251.

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Abstract

Adult Teladorsagia circumcincta survival and motility in vitro was examined in a range of different cell culture media, supplements and gas mixes. Under optimum conditions, worms survived for 14 days, exhibiting high motility for 9 days and egg production for 72 h. Optimum conditions involved co-culture of worms with a HeLa cell line in a supplemented cell medium (CEM) and an atmosphere containing 10% CO2, 5% O2 85% N2, 65% humidity at 37 °C. The incubation medium consisted of Minimum Essential Medium with 10% fetal calf serum, 1% non-essential amino acids, 1% glutamax and 1% penicillin–neomycin–streptomycin cocktail mix. Compared with optimum conditions, incubation in CEM alone, cell conditioned CEM, RPMI alone, Medium 199 alone, reduced CO2 or O2, or when cells were replaced with Escherichia coli, both survival and motility were reduced. Optimum conditions for adult T. circumcincta maintenance for culture, anthelmintic testing or generation of excretory/secretory products are described.

Item ID: 35757
Item Type: Article (Research - C1)
ISSN: 0014-4894
Keywords: Teladorsagia circumcincta, nematodes, in vitro culture, cell tissue culture, in vitro egg laying
Funders: Meat and Wool New Zealand, Agricultural and Marketing Research and Development Trust (AGMARDT)
Date Deposited: 15 Oct 2014 16:48
FoR Codes: 06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060104 Cell Metabolism @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 50%
97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 50%
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