In vitro sensitivity of the amphibian pathogen batrachochytrium dendrobatidis to antifungal therapeutics

Woodward, A., Berger, L., and Skerratt, L.F. (2014) In vitro sensitivity of the amphibian pathogen batrachochytrium dendrobatidis to antifungal therapeutics. Research in Veterinary Science, 97 (2). pp. 364-366.

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Abstract

Chytridiomycosis, a skin disease caused by Batrachochytrium dendrobatidis, has caused amphibian declines worldwide. Amphibians can be treated by percutaneous application of antimicrobials, but knowledge of in vitro susceptibility is lacking. Using a modified broth microdilution method, we describe the in vitro sensitivity of two Australian isolates of B. dendrobatidis to six antimicrobial agents. Growth inhibition was observed, by measurement of optical density, with all agents. Minimum inhibitory concentrations (μg/mL; isolate 1/2) were - voriconazole 0.016/0.008; itraconazole 0.032/0.016; terbinafine 0.063/0.063; fluconazole 0.31/0.31; chloramphenicol 12.5/12.5; amphotericin B 12.5/6.25. Killing effects on zoospores were assessed by observing motility. Amphotericin B and terbinafine killed zoospores within 5 and 30 min dependent on concentration, but other antimicrobials were not effective at the highest concentrations tested (100 μg/mL). This knowledge will help in drug selection and treatment optimization. As terbinafine was potent and has rapid effects, study of its pharmacokinetics, safety and efficacy is recommended.

Item ID: 35214
Item Type: Article (Research - C1)
ISSN: 1532-2661
Keywords: Batrachochytrium dendrobatidis; antifungal testing; treatment; chytridiomycosis
Funders: Australian Research Council (ARC), James Cook University (JCU)
Date Deposited: 10 Oct 2014 01:35
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070707 Veterinary Microbiology (excl Virology) @ 50%
07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070710 Veterinary Pharmacology @ 50%
SEO Codes: 96 ENVIRONMENT > 9608 Flora, Fauna and Biodiversity > 960805 Flora, Fauna and Biodiversity at Regional or Larger Scales @ 100%
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