The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA

Yang, Xiao, Molimau, Seeseei, Doherty, Geoff P., Johnston, Elecia B., Marles-Wright, Jon, Rothnagel, Rosalba, Hankamer, Ben, Lewis, Richard J., and Lewis, Peter J. (2009) The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA. EMBO Reports, 10 (9). pp. 997-1002.

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There are three stages of transcribing DNA into RNA. These stages are initiation, elongation and termination, and they are well-understood biochemically. However, despite the plethora of structural information made available on RNA polymerase in the last decade, little is available for RNA polymerase in complex with transcription elongation factors. To understand the mechanisms of transcriptional regulation, we describe the first structure, to our knowledge, for a bacterial RNA polymerase in complex with an essential transcription elongation factor. The resulting structure formed between the RNA polymerase and NusA from Bacillus subtilis provides important insights into the transition from an initiation complex to an elongation complex, and how NusA is able to modulate transcription elongation and termination.

Item ID: 35123
Item Type: Article (Research - C1)
ISSN: 1469-3178
Keywords: Bacillus subtilis; NusA; RNA polymerase; single particle analysis; transcription
Funders: Australian Research Council (ARC), Biotechnology and Biological Sciences Research Council (BBSRC), Australian Postgraduate Award
Projects and Grants: ARC DP0664370, ARC DP0452362, ARC LX056108
Date Deposited: 02 Oct 2014 04:31
FoR Codes: 03 CHEMICAL SCIENCES > 0304 Medicinal and Biomolecular Chemistry > 030403 Characterisation of Biological Macromolecules @ 20%
06 BIOLOGICAL SCIENCES > 0605 Microbiology > 060501 Bacteriology @ 30%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060112 Structural Biology (incl Macromolecular Modelling) @ 50%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 100%
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