Eimeria maxima: ELISA and Western blot analyses of protective sera

Wallach, Michael, Smith, Nicholas C., Miller, Catherine M.D., Eckert, Johannes, and Rose, M. Elaine (1994) Eimeria maxima: ELISA and Western blot analyses of protective sera. Parasite Immunology, 16 (7). pp. 377-383.

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Infection of chickens with Eimeria maxima induces the production of parasite-specific antisera which can be used passively to protect naïve chickens against infection. Globulin fractions of these antisera can also be used passively to protect chickens. Similarly, intramuscular injection of soybean lectin affinity purified gametocyte antigens of E. maxima in Freund's Complete Adjuvant induces production of antibodies which are maternally transferred and thereby protect hatchlings against E. maxima. ELISA analyses of serum pools having varying protective capacities revealed good correlations between passive protection and levels of anti-unsporulated oocyst, anti-sporulated oocyst, anti-merozoite and anti-gametocyte antibodies. Western blotting demonstrated that the sera mainly recognized a number of high molecular weight antigens in all developmental stages and that the intensity of the reactions reflected the degree of protection induced by the sera. Sera from birds immunized with gametocyte antigens also recognized high molecular weight antigens from all the developmental stages, with banding patterns remarkably similar to those observed for sera from infected birds. Taken together, these results indicate that antibodies can protect against infection with E. maxima and these antibodies may recognize and act against asexual and/or sexual stages of the parasite.

Item ID: 34618
Item Type: Article (Research - C1)
ISSN: 1365-3024
Keywords: E. maxima; coccidiosis; stage-specificity; ELISA, western blotting, antibodies; immunity
Funders: Bundesamt für Bildung und Wissenschaft, Bern, Bundesamt für Veterinarwesen, Liebefeld-Bern, Swiss National Science Foundation, Roche Foundation
Projects and Grants: Bundesamt für Bildung und Wissenschaft (Coccidiosis, COST 89), Bundesamt für Veterinarwesen ( 012.92.8), Swiss National Science Foundation (31-35656.92)
Date Deposited: 26 Aug 2014 06:05
FoR Codes: 07 AGRICULTURAL AND VETERINARY SCIENCES > 0707 Veterinary Sciences > 070708 Veterinary Parasitology @ 70%
06 BIOLOGICAL SCIENCES > 0601 Biochemistry and Cell Biology > 060199 Biochemistry and Cell Biology not elsewhere classified @ 30%
SEO Codes: 97 EXPANDING KNOWLEDGE > 970107 Expanding Knowledge in the Agricultural and Veterinary Sciences @ 70%
97 EXPANDING KNOWLEDGE > 970106 Expanding Knowledge in the Biological Sciences @ 30%
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